Actinomyces Israelii

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Actinomyces israelii

Morphology

• Size, shape and motility o Actinomyces israelii is small, filamentous bacteria. It is a bacillus and is normally seen in short chains. Actinomyces is non motile.3 o Some cultures may produce colonies made up of short angular branching filaments or small rough compact colonies with no filaments 6 o Macrocolonies on blood agar and Brain- Heart infusion agar are .5-3 mm in diameter. These colonies are circular to irregular, lobate and white.6 o Structures called fimbriae on the cell surface that are similar to, but smaller than flagella, and made of protein located on the bacterial cell surface allow A. israelii to grow in biofilms.3
• Genetics o The genome of A. israelii is currently being sequenced, using the Actinomyces israelii F0345 strain for comparative genome analysis.3 o Actinobacteria are categorized by their high GC content in DNA, about 60%. 6
• Cell envelope & sporulation o Actinomyces israelii species is gram-positive, cast-forming, non–acid-fast, and non–spore-forming. 8
• Mode of reproduction o Slow-growing by binary fission. On an enriched solid medium such as brain-heart infusion agar in an environment of 37°C. 11
• Specimen collection o A specimen is collected from pus aspirated from abscess (usually in the neck or pelvic area) and is incubated for a minimum of 5 days. 4
• Identification o The most effective way to identify is by Gram stain. Specimen stains purple for gram positive. 4 o The gram stain should also reveal its filamentous nature.
• Diagnosis of disease o Actinomyces israelii frequently causes Actinomycosis.2 o Pus tests and biopsys may be done to test for Actinomyces israelii.
Transmission
• Ecological niche o Actinomyces israelii is carried by humans. It is a natural component of the human gastrointestinal and oral flora. 3 o It is also commonly found in soil. A.…...

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