Enzyme Lab

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Enzymatic Reaction of Sucrose and Sucrase: Analysis Under Different Conditions and Concentrations


Sucrase is the enzyme that breaks down sucrose into glucose and fructose. The purpose of this lab experiment was to determine under what environment the enzymatic reaction between sucrose and sucrase would produce the most products and the rate of production.
To determine the rate of reaction, Benedicts Reagent was used to identify the amount of glucose produced from the enzymatic reaction. Benedicts Reagent is used to detect the presence of glucose and indicates the results with varying degrees of color.
We were successful in our endeavors to measure this rate of reaction with Benedicts Reagent and conclude that the higher the substrate or enzyme concentration, the faster the rate. The process of using Benedicts Reagent to measure glucose levels is also used in urine analysis for people with diabetes.

All living organisms need to supply themselves with nutrients and as humans, we use the process of digestion to break down and extrapolate the nutrients from our food to maintain and fuel our bodies. In order to perform digestion our bodies use enzymes, which are biological catalysts. They are made of proteins that responsible for the chemical reactions essential to sustaining life. Enzymes have three major characteristics: increase the rate of reaction, are substrate specific and lower the energy barrier it takes to for reactants to occur. Enzymes can also react differently under certain conditions and concentration levels, being the most productive at their specific condition and concentration.
Proteins become enzymes when they bind to and react with a molecule, which is called substrate. Each enzyme has a specific function and only bind its specific substrate. In this lab experiment we used the enzyme sucrase, extracted…...

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...------------------------------------------------- Enzyme From Wikipedia, the free encyclopedia   (Redirected from Enzymology) Enzymes /ˈɛnzaɪmz/ are macromolecular biological catalysts. Enzymes accelerate, or catalyze, chemical reactions. The molecules at the beginning of the process are called substrates and the enzyme converts these into different molecules, called products. Almost all metabolic processes in the cell need enzymes in order to occur at rates fast enough to sustain life.[1]:8.1 The set of enzymes made in a cell determines which metabolic pathways occur in that cell. The study of enzymes is called enzymology. Enzymes are known to catalyze more than 5,000 biochemical reaction types.[2] Most enzymes are proteins, although a few are catalytic RNA molecules. Enzymes' specificity comes from their uniquethree-dimensional structures. Like all catalysts, enzymes increase the rate of a reaction by lowering its activation energy. Some enzymes can make their conversion of substrate to product occur many millions of times faster. An extreme example is orotidine 5'-phosphate decarboxylase, which allows a reaction that would otherwise take millions of years to occur in milliseconds.[3][4] Chemically, enzymes are like any catalyst and are not consumed in chemical reactions, nor do they alter the equilibrium of a reaction. Enzymes differ from most other catalysts by being much more specific. Enzyme activity can be affected by other molecules: inhibitors are molecules......

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