Enzyme Lab

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Enzymatic Reaction of Sucrose and Sucrase: Analysis Under Different Conditions and Concentrations

Abstract:

Sucrase is the enzyme that breaks down sucrose into glucose and fructose. The purpose of this lab experiment was to determine under what environment the enzymatic reaction between sucrose and sucrase would produce the most products and the rate of production.
To determine the rate of reaction, Benedicts Reagent was used to identify the amount of glucose produced from the enzymatic reaction. Benedicts Reagent is used to detect the presence of glucose and indicates the results with varying degrees of color.
We were successful in our endeavors to measure this rate of reaction with Benedicts Reagent and conclude that the higher the substrate or enzyme concentration, the faster the rate. The process of using Benedicts Reagent to measure glucose levels is also used in urine analysis for people with diabetes.

Introduction:
All living organisms need to supply themselves with nutrients and as humans, we use the process of digestion to break down and extrapolate the nutrients from our food to maintain and fuel our bodies. In order to perform digestion our bodies use enzymes, which are biological catalysts. They are made of proteins that responsible for the chemical reactions essential to sustaining life. Enzymes have three major characteristics: increase the rate of reaction, are substrate specific and lower the energy barrier it takes to for reactants to occur. Enzymes can also react differently under certain conditions and concentration levels, being the most productive at their specific condition and concentration.
Proteins become enzymes when they bind to and react with a molecule, which is called substrate. Each enzyme has a specific function and only bind its specific substrate. In this lab experiment we used the enzyme sucrase, extracted…...

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