Lab Isolation

In: Other Topics

Submitted By progers
Words 1233
Pages 5
Abstract
The purpose of this exercise to become familiar with subtypes of culture media, to learn how to use streak and pour techniques and to generate a pure culture of a specific organism Hypothesis
This exercise will allow me to gain an understanding of culture media, to use specific techniques such as streak and pour and generate cultures of specific organisms.
Procedures
Exercise 1
Part I
1. Disinfect the work area.
2. Melt the agar tubes.
3. Leave the 18 mL tube of MRS agar in hot water (50°C) for use in Part II.
4. Use the marking pencil to label the bottom of one Petri dish S. epidermidis. Pour one half (9mL) of the contents of a tube of nutrient agar into the S. epidermidis Petri dish and the other half into the bottom of an unmarked Petri dish. Cover the dishes and allow them to solidify for use in Part IV.
5. Pour the remaining melted nutrient agar into the unmarked Petri dishes (half a tube per dish). Cover the dishes and allow them to solidify for use in Part III.

Part II
1. Disinfect the work area.
2. Label the bottom surface of three sterile Petri dishes L. acidophilus #1, #2, and #3, respectively. 3. Disinfect three test tubes by submerging them in boiling water for 5 minutes. The tubes will be hot, so use tongs or tweezers to lift them out of the water. Be careful not to contaminate the tubes by touching their lips or interiors. When the tubes are cool, label them to match the L. acidophilus Petri dishes.
4. Divide the liquid MRS agar into the three test tubes marked L. acidophilus. If the agar has begun to solidify, reheat it until it is fully melted. Set the test tubes of agar in the hot water to prevent them from solidifying.
5. After ensuring the tubes of agar are cool enough not to kill the bacterial culture but are still fully liquid, use aseptic techniques to inoculate the tube labeled L.…...

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